Key to successful 3D cell culturing is to have a scaffold that allows cells to utilize both their cell-cell (cadherin) and cell-matrix (integrin) receptors. This is how cells grow in vivo and communicate with the other cells and the extracellular matrix.
SeedEZ is a robust 3D cell culture scaffold that enables cells to do this. Place the scaffold into your favorite dish or plate, add cells or cells in an extracellular matrix and continue with your routine protocols with the benefit of physiologically relevant cell behavior in vitro.