AzuraQuant™ Probe 1-Step qRT-PCR Kit LoRox, Optimized for COVID-19 Testing, 20 x 1mL, 1000 rxns

The AzuraQuant™ Probe One-Step qPCR Mix is a ready-to-use 2x master mix and companion thermostable reverse transcriptase for use in highly sensitive real-time RT-PCR assays and has been formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes.

The AzuraQuant™ Probe One-Step qPCR Mix is powered by Azura HS Taq DNA Polymerase, AzuraSprint Reverse Transcriptase, and an optimized buffer chemistry, providing robust first-strand cDNA synthesis and real-time PCR in a single tube. The Mix delivers earlier quantification cycle values (Ct) and broad range detection for increased sensitivity, speed, reliability and reproducibility.

The AzuraQuant™ Probe One-Step qPCR Mix can be used to quantify a specific target RNA from either total RNA or mRNA while reducing the number of pipetting steps and time to result. The AzuraQuant™ Probe One-Step qPCR Mix requires little if any optimization and employs rapid antibody-mediated hot-start activation and a processive enzyme chemistry, providing unmatched performance in muliplexing.

Applications

• Multiplex RT-qPCR
• Gene Expression analysis
• Pathogen detection
• Viral Quantitation
• Biomarker discovery
• Gene knock-down validation
• Microarray validation and Detection of extremely low copy targets

Flexibility
Compatible with standard and fast cycling instruments and a wide range of cycling parameters.

Accurate Quantification
Hot-Start chemistry reduces primer-dimer formation and allows room-temperature assembly.

Superior Multiplex Capability
Advanced formulation provides unrivalled sensitivity in singleplex as well as complex multiplex assays.