Introduction

ViraTrap^TM adenovirus associate virus purification kit is a fast AAV purification method with high recovery. The AAV can be purified in 60-120 minutes while the traditional CsCl ultracentrifugation takes 24 hours.

The Kit provides 5 columns for 10 preparations and each preparation can produce up to 1x 10¹³ Vps. The purified virus can be used for infection of the cells and animals.

Up to 1 x10¹² viral particles can be purified from cell culture media of 1 to 2 T75 flasks.

Each column can be regenerated for purifying the same rAAV. For optimized viral binding and recovery, each column can be regenerated only once.

Includes: 

Regeneration Buffer 100 x Nuclease, Reaction BufferNuclease, Binding Buffer, 15 mL Collection Tube, Desalting Tube, Press-On Cap, Mini Columns

Storage and Stability

All components are guaranteed for at least 12 months from the date of purchase.

Safety Considerations

The adenovirus infected cell media and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.

Materials Required But Not Supplied 

1. ddH₂O
2. PBS
3. 0.45 μm and 0.22 μm filters
4. Rack holder for columns