The yield of plasmid DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high copy number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 3 to 5 times

Materials supplied by user

•  Centrifuge with swing-bucket rotor (4,000 x g).
•  Please disinfect 96-well 2.2 mL plates before use.
•  Vacuum pump capable of achieving 300-400 mbar.
•  Standard vacuum manifold.
•  Oven or incubator preset to 70 oC.

Storage and Stability

All components are guaranteed for 24 months from the date of purchase. The Buffer A1/RNase A should be stored at 4oC.

Kits include all the deep well plates, sealing films, and reagents required to perform 20 extractions using 96 well plates