Our Protease Inhibitor Cocktail, His-Tag [100X] has a broad specificity for the inhibition of serine, cysteine, acid, thermolysin-like proteases and aminopeptidases and metalloproteases.

Cell extracts for Histidine-Tagged (His-Tag) protein contain a number of endogenous proteases, that are capable of modifying the proteins present in the extract/lysate.

The need for protease inhibitors arises to protect the proteins from damage caused by these proteases released during the cellular lysis.

Also, to improve the yield of native proteins, use of protease inhibitor cocktail along with phosphatase and other inhibitors is recommended during the extraction process.

Our Histidine-Tagged (His-Tag) protease inhibitor cocktail contains optimized concentration of protease inhibitors: AEBSF (4-[2-Aminoethyl] benzenesulfonyl fluoride hydrochloride), Bestatin, Pepstatin A, E-64 (N-[trans-Epoxysuccinyl]-L-leucine 4-guanidinobutylamide), Phosphoramidon protease inhibitors and other proprietary component(s) for broad spectrum inhibition of proteases that target serine proteases (e.g., trypsin, chymotrypsin, plasmin, kallikrein, and thrombin), cysteine proteases (e.g., calpain, papain, cathepsin B, and cathepsin L), aminopeptidases (e.g., leucine aminopeptidase and alanyl aminopeptidase, acid proteases (e.g., pepsin, rennin, and cathepsin D, and many microbial aspartic proteases), and membrane metallo-endopeptidase (e.g., thermolysin and collagenase), etc.

Our Histidine-Tagged (His-Tag) protease inhibitor cocktail does not contain any chelators, such as EDTA, to optimize its use in the purification of histidine-tagged proteins and is compatible with immobilized metal affinity chromatography.

Our Histidine-Tagged (His-Tag) protease inhibitor cocktail is supplied as a ready to use solution in DMSO, at a 100X concentration, which makes it easier to use at lower volumes at 1X, 2X or more for samples with high protease activities.

Our Histidine-Tagged (His-Tag) protease inhibitor cocktail preserves proteins from degradation by proteases and can be used with the lysis buffer for His-Tag protein extractions or lysate.

Our protease inhibitor cocktails can be ship at room temperature, but should be stored at 4C for longevity