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Accuris qMAX™ Green One-Step RT-qPCR Kit allows for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates.
Workflows are simplified by the one tube formulation of ready-to-use 2X qPCR master mix and reverse transcriptase.
The optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLVderived reverse transcriptase for robust first strand cDNA synthesis.
Accuris Hot Start Taq Polymerase is the engine for qPCR, while qMAX Green allows for dye based detection. qMAX Green™ exhibits greater fluorescence upon binding to double stranded DNA than other green dyes while exhibiting minimal PCR inhibition. An inert blue dye is included in the mix to help simplify pipetting and reduce errors.
The Accuris qMAX Green One-Step qPCR Kit is compatible with standard and fast cycling protocols and provides increased sensitivity, speed and reproducibility for a broad range of samples and targets.
The polymerase mix is available with low or high level of ROX reference dye for compatibility with all qPCR instruments.
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