Radiant HiFi Ultra Polymerase, High Fidelity / Proofreading, including dNTP's, 2units/µl concentration, 1000 units

Ultra-High Fidelity Including dNTP's

• >50-fold higher fidelity than standard Taq DNA Polymerase
• New-generation PCR buffer formulation for maximum PCR efficiency and reaction speed.
• Robust PCR performance across a wide range of DNA templates including genomic DNA and GC-rich and AT-rich sequences.
• Supplied with 5X HiFi Ultra Reaction Buffer containing MgCl2 and highly pure dNTPS.
• High DNA yields with either fast cycling or standard cycling.

Radiant™ HiFi Ultra DNA Polymerase is a high-performance, high-fidelity DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA.

Radiant™ HiFi Ultra Polymerase possesses 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities with an error-rate of 4.55 x 10-7 . The polymerase is ideal for cloning applications, library construction, screening and genotyping of problematic GC-rich and AT-rich sequences.

Radiant™ HiFi Ultra Polymerase is engineered for robust, superior high-fidelity PCR in comparison with standard proof-reading enzymes such as pfu DNA Polymerase. Several point-mutations combined with a new-generation buffer formulation provide for significantly improved processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples.

The supplied 5X HiFi Ultra Reaction Buffer contains an ideal concentration of MgCl2 and highly pure dNTPS for convenience and consistency in liquid handling. 

Important Considerations:

Radiant™ 5x HiFi Ultra Reaction Buffer: The 5x reaction buffer contains 15mM MgCl2, 5mM dNTPs, and PCR enhancers. The buffer
composition has been optimized for maximum efficiency, sensitivity and success with difficult amplicons. We do not suggest the use of additional MgCl2 or enhancers.

Template: For complex genomic DNA, we suggest the use of 5ng - 500ng per reaction; For cDNA or plasmid DNA, please use < 100ng per
reaction.

Primers: Primers should have a predicted melting temperature of around 60°C, using default Primer 3 settings (http://frodo.wi.mit.edu/primer3/). The final primer concentration in the reaction should be between 0.2μM and 0.6μM.

Annealing: We recommend performing a temperature gradient to determine the optimal annealing temperature. Alternatively, we suggest
a 57°C annealing temperature and then an increase in 2°C increments if non-specific products are present.

Extension: Optimal extension is achieved at 72°C. The optimal extension time is dependent on amplicon length and complexity.
30 seconds per kilobase(Kb) is recommended for amplification from eukaryotic genomic DNA or cDNA.