![RIPA-Lysis-buffer-For Protein Extraction And Stabilization From Adherent or Suspension Cells - Proteomic Supplies - Stellar Scientific RIPA-Lysis-buffer-For Protein Extraction And Stabilization From Adherent or Suspension Cells - Proteomic Supplies - Stellar Scientific](https://cdn11.bigcommerce.com/s-w9bdixgj/images/stencil/500x659/products/6075/12598/RIPA-Lysis-buffer-For_Protein_Extraction_And_Stabilization_From_Adherent_or_Suspension_Cells_-_Proteomic_Supplies_-_Stellar_Scientific__42410.1690738330.jpg?c=2)
![RIPA-Lysis-buffer-For Protein Extraction And Stabilization From Adherent or Suspension Cells - Proteomic Supplies - Stellar Scientific RIPA-Lysis-buffer-For Protein Extraction And Stabilization From Adherent or Suspension Cells - Proteomic Supplies - Stellar Scientific](https://cdn11.bigcommerce.com/s-w9bdixgj/images/stencil/500x659/products/6075/12598/RIPA-Lysis-buffer-For_Protein_Extraction_And_Stabilization_From_Adherent_or_Suspension_Cells_-_Proteomic_Supplies_-_Stellar_Scientific__42410.1690738330.jpg?c=2)
The buffer is very effective in extracting cytoplasmic, nuclear and membrane proteins and the time for protein extraction takes about 60 minutes.
The RIPA lysis buffer contains non-ionic and ionic detergents, that allows extraction of proteins from different cell types and membrane structures.
The supplied RIPA buffer contains 1% NP-40, 1% sodium deoxycholate, 0.1% SDS with 25mM Tris and 150mM NaCl.
RIPA buffer ensures efficient cell lysis and protein solubilization preventing protein degradation and interference with protein immunoreactivity and biological activity.
Most antibodies and protein antigens are not adversely affected by the components of the RIPA Buffer.
RIPA buffer minimizes non-specific protein-binding interactions and keep low background, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions.
The RIPA Lysis Buffer is used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
Our RIPA Lysis Buffer is highly effective and efficient for lysis and protein solubilization of wide range of samples, including mammalian cell and cover membranous, cytoplasmic, and nuclear protein extractions. It enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity.
RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays.
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