SMOBio Champion E. coli Transformation Kit, 200 rxn
SMOBio Champion E. coli Transformation Kit, 200 rxn
- SKU:
- SMOB-20-200
- Weight:
- 5.00 LBS
- Shipping:
- Calculated at Checkout
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Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate.
The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year.
This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently.
Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains.
The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps.
Features
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Flexible– fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation.
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Fast and Easy– only few steps for preparation; suitable for time-saving transformation
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High efficiency– up to 109 cfu/μg
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Personalization– suitable for most E. coli strains
Kit contains:
Champion™ CC Buffer - 20mL
SMO-Broth™ - 2 x 100mL
pUC19 Control Plasmid (10-4 μg/μl) - 5μl
Instruction manual and Champion™ Competent Cell Preparation Card
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Prepare Competent Cells with High Transformation Efficiency Using Champion Kit
http://www.smobio.com/Product-clonings-ck1000.html Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. The resultant competent cells can be stored at -70°C for one year or be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains.
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Prepare Competent Cells Directly from E.coli Colonies Using Champion E.coli Transformation Kit
Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells directly from bacterial colonies on the plate. http://www.smobio.com/Product-clonings-ck1000.html Instant Protocol: 1. Gently collect and mix five E. coli colonies (diameter ~1 mm2) into 100 μl pre-cooled SMO-Broth™. 2. Harvest the E. coli with 4000 x g for 5 min at 4°C. 3. Remove the medium and gently resuspend the E. coli in 100 μl pre-cooled Champion™ CC Buffer. The E. coli suspension is ready for transformation
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